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wild type l plantarum strains  (ATCC)
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ATCC wild type l plantarum strains
Initial rates of cadmium uptake by E. coli and L. <t> plantarum </t> in APT medium a
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Initial rates of cadmium uptake by E. coli and L. plantarum in APT medium a

Journal:

Article Title: Cloning, Expression, and Characterization of Cadmium and Manganese Uptake Genes from Lactobacillus plantarum

doi:

Figure Lengend Snippet: Initial rates of cadmium uptake by E. coli and L. plantarum in APT medium a

Article Snippet: To identify MntA expression, crude cell extracts or total membrane proteins of two wild-type L. plantarum strains (ATCC 14917 and ATCC 8014) as well as two Mn 2+ -dependent mutants (mnd15-26 and mnd11-06) were prepared, separated by SDS-PAGE, and immunoblotted with an MntA antibody.

Techniques:

Induction of the synthesis of mntA mRNA by Mn2+ starvation in L. plantarum ATCC 14917. Total RNA was isolated from L. plantarum ATCC 14917 Mn2+-starved (−) or Mn2+-sufficient (+) cells, treated with DNase I, and reverse transcribed with a specific antisense primer. The RT reaction mixture was used as a template to perform PCR to amplify a 1.1-kb fragment of mntA as described in the text. Each lane represents a separate experiment with four independent RNA preparations. MW, molecular weight.

Journal:

Article Title: Cloning, Expression, and Characterization of Cadmium and Manganese Uptake Genes from Lactobacillus plantarum

doi:

Figure Lengend Snippet: Induction of the synthesis of mntA mRNA by Mn2+ starvation in L. plantarum ATCC 14917. Total RNA was isolated from L. plantarum ATCC 14917 Mn2+-starved (−) or Mn2+-sufficient (+) cells, treated with DNase I, and reverse transcribed with a specific antisense primer. The RT reaction mixture was used as a template to perform PCR to amplify a 1.1-kb fragment of mntA as described in the text. Each lane represents a separate experiment with four independent RNA preparations. MW, molecular weight.

Article Snippet: To identify MntA expression, crude cell extracts or total membrane proteins of two wild-type L. plantarum strains (ATCC 14917 and ATCC 8014) as well as two Mn 2+ -dependent mutants (mnd15-26 and mnd11-06) were prepared, separated by SDS-PAGE, and immunoblotted with an MntA antibody.

Techniques: Isolation, Reverse Transcription, Molecular Weight

Identification of MntA in wild-type L. plantarum strains (A) and Mn2+-dependent mutants (B). Western blotting was done with an MntA antibody and protein extracts from L. plantarum strains and Cd2+ uptake mutants. (A) Lane 1, molecular mass standards (in kilodaltons); lanes 2 and 5, membrane protein from Mn2+-starved cells of ATCC 8014 and ATCC 14917, respectively; lanes 3 and 6, total protein from Mn2+-starved cells of ATCC 8014 and ATCC 14917, respectively; lanes 4 and 7, total protein from Mn2+-sufficient cells of ATCC 8014 and ATCC 14917, respectively. (B) Lanes 1 and 6, total protein from Mn2+-starved cells of ATCC 8014 and ATCC 14917, respectively; lanes 2 and 4, total protein from Mn2+-starved cells of mnd15-26 and mnd11-06, respectively; lanes 3 and 7, molecular weight standards; lane 5, membrane protein from Mn2+-starved cells of ATCC 8014.

Journal:

Article Title: Cloning, Expression, and Characterization of Cadmium and Manganese Uptake Genes from Lactobacillus plantarum

doi:

Figure Lengend Snippet: Identification of MntA in wild-type L. plantarum strains (A) and Mn2+-dependent mutants (B). Western blotting was done with an MntA antibody and protein extracts from L. plantarum strains and Cd2+ uptake mutants. (A) Lane 1, molecular mass standards (in kilodaltons); lanes 2 and 5, membrane protein from Mn2+-starved cells of ATCC 8014 and ATCC 14917, respectively; lanes 3 and 6, total protein from Mn2+-starved cells of ATCC 8014 and ATCC 14917, respectively; lanes 4 and 7, total protein from Mn2+-sufficient cells of ATCC 8014 and ATCC 14917, respectively. (B) Lanes 1 and 6, total protein from Mn2+-starved cells of ATCC 8014 and ATCC 14917, respectively; lanes 2 and 4, total protein from Mn2+-starved cells of mnd15-26 and mnd11-06, respectively; lanes 3 and 7, molecular weight standards; lane 5, membrane protein from Mn2+-starved cells of ATCC 8014.

Article Snippet: To identify MntA expression, crude cell extracts or total membrane proteins of two wild-type L. plantarum strains (ATCC 14917 and ATCC 8014) as well as two Mn 2+ -dependent mutants (mnd15-26 and mnd11-06) were prepared, separated by SDS-PAGE, and immunoblotted with an MntA antibody.

Techniques: Western Blot, Membrane, Molecular Weight